产品目录

本品为来源于兔的抗ERK1的多克隆抗体。
宿主：兔
克隆性：多克隆抗体
用途：WB,ICC, IHC,IF
交叉反应性：人，小鼠，大鼠
分子量：44KDa
推荐稀释比例：WB(1:500-1:1000),IF(1:200),IHC(1:200),ICC(1:200)

About this Antibody
Name	ERK1 Rabbit Polyclonal Antibody
Category	Polyclonal antibody(pAb); Primary antibody
Isotype	IgG
Purification	Peptide affinity purified
About the Immunogen
Immunogen	This antibody is produced by immunizing rabbits with synthetic peptide corresponding to the C-terminal of ERK1.
Gene ID	5595
SwissProt	P27361
Synonyms	Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associated protein 2 kinase; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kin
Category	MAP Kinase Signaling
Background	Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.

注意事项：
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
2. 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。

储存条件：-20℃，有效期一年。
相关搜索：兔抗ERK1抗体，ERK1 Rabbit Polyclonal Antibody